| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Binding to PCNA in Euryarchaeal DNA Replication Requires Two PIP Motifs for DNA Polymerase D and One PIP Motif for DNA Polymerase B
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| Autores: |
Castrec, Benoit
Rouillon, Christophe
Henneke, Ghislaine
Flament, Didier
Querellou, Joel
Raffin, Jean-paul
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| Data: |
2009-11
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| Ano: |
2009
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| Palavras-chave: |
Archaea
PIP box
PCNA binding motifs
DNA polymerases
DNA replication
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| Resumo: |
Replicative DNA polymerases possess a canonical C-terminal proliferating cell nuclear antigen (PCNA)-binding motif termed the PCNA-interacting protein (PIP) box. We investigated the role of the PIP box on the functional interactions of the two DNA polymerases, PabPol B (family B) and PabPol D (family D), from the hyperthermophilic euryarchaeon Pyrococcus abyssi, with its cognate PCNA. The PIP box was essential for interactions of PabPol B with PCNA, as shown by surface plasmon resonance and primer extension studies. In contrast, binding of PabPol D to PCNA was affected only partially by removing the PIP motif. We identified a second palindromic PIP box motif at the N-terminus of the large subunit of PabPol D that was required for the interactions of PabPol D with PCNA. Thus, two PIP motifs were needed for PabPol D for binding to PabPCNA. Moreover, the C-terminus of PabPCNA was essential for stimulation of PabPol D activity but not for stimulation of PabPol B activity. Neither DNA polymerase interacted with the PabPCNA interdomain connecting loop. Our data suggest that distinct processes are involved in PabPol D and PabPol B binding to PCNA, raising the possibility that Archaea require two mechanisms for recruiting replicative DNA polymerases at the replication fork. (C) 2009 Elsevier Ltd. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2009/publication-7317.pdf
DOI:10.1016/j.jmb.2009.09.044
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/7317/
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| Formato: |
application/pdf
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| Fonte: |
Journal of Molecular Biology (0022-2836) (Elsevier), 2009-11 , Vol. 394 , N. 2 , P. 209-218
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| Direitos: |
2009 Elsevier B.V. All rights reserved.
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